LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1694..1790) /label=attL2 /note="attL2 (c)" misc_signal complement(1805..1824) /note="T7 Promoter/priming site (c)" primer_bind 1832..1848 /note="M13 Reverse priming site" gene 1961..2770 /note="Kanamycin resistance gene" rep_origin 2891..3564 /note="pUC origin" vector join(1702..3567,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" CDS 692..1687 /dnas_title="ST6 beta-galactosamide alpha-2,6-sialyltranferase 1" /gene="ST6GAL1" /gene_synonym=CD75 /gene_synonym=MGC48859 /gene_synonym=ST6Gal I /gene_synonym=ST6GalI /codon_start=1 /product="ST6 beta-galactosamide alpha-2,6-sialyltranferase 1" /protein_id="AAH31476.1" /db_xref="GI:45708716" /db_xref="GeneID:6480" /db_xref="HGNC:10860" /db_xref="MIM:109675" /translation="MIHTNLKKKFSCCVLVFLLFAVICVWKEKKKGSYYDSFKLQTKE FQVLKSLGKLAMGSDSQSVSSSSTQDPHRGRQTLGSLRGLAKAKPEASFQVWNKDSSS KNLIPRLQKIWKNYLSMNKYKVSYKGPGPGIKFSAEALRCHLRDHVNVSMVEVTDFPF NTSEWEGYLPKESIRTKAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNGAPTAN FQQDVGTKTTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQNPDYN FFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGMLGIIIMMTL CDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEKNLVKHLNQGTDEDIYL LGKATLPGFRTIHC" source 692..1687 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17105 IMAGE:4339124" /tissue_type="Lymph, lymphoma" /clone_lib="NIH_MGC_85" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1687 /gene="ST6GAL1" /gene_synonym=CD75 /gene_synonym=MGC48859 /gene_synonym=ST6Gal I /gene_synonym=ST6GalI /db_xref="GeneID:6480" /db_xref="HGNC:10860" /db_xref="MIM:109675" misc_feature 692..1687 /note="ST6Gal1" misc_difference 1306..1306 /gene="ST6GAL1" /gene_synonym=CD75 /gene_synonym=MGC48859 /gene_synonym=ST6Gal I /gene_synonym=ST6GalI /note="'T' in cDNA is 'C' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccgccagacc ctcggcagtc tcagaggcct 721 agccaaggcc aaaccagagg cctccttcca ggtgtggaac aaggacagct cttccaaaaa 781 ccttatccct aggctgcaaa agatctggaa gaattaccta agcatgaaca agtacaaagt 841 gtcctacaag gggccaggac caggcatcaa gttcagtgca gaggccctgc gctgccacct 901 ccgggaccat gtgaatgtat ccatggtaga ggtcacagat tttcccttca atacctctga 961 atgggagggt tatctgccca aggagagcat taggaccaag gctgggcctt ggggcaggtg 1021 tgctgttgtg tcgtcagcgg gatctctgaa gtcctcccaa ctaggcagag aaatcgatga 1081 tcatgacgca gtcctgaggt ttaatggggc acccacagcc aacttccaac aagatgtggg 1141 cacaaaaact accattcgcc tgatgaactc tcagttggtt accacagaga agcgcttcct 1201 caaagacagt ttgtacaatg aaggaatcct aattgtatgg gacccatctg tataccactc 1261 agatatccca aagtggtacc agaatccgga ttataatttc tttaataact acaagactta 1321 tcgtaagctg caccccaatc agccctttta catcctcaag ccccagatgc cttgggagct 1381 atgggacatt cttcaagaaa tctccccaga agagattcag ccaaaccccc catcctctgg 1441 gatgcttggt atcatcatca tgatgacgct gtgtgaccag gtggatattt atgagttcct 1501 cccatccaag cgcaagactg acgtgtgcta ctactaccag aagttcttcg atagtgcctg 1561 cacgatgggt gcctaccacc cgctgctcta tgagaagaat ttggtgaagc atctcaacca 1621 gggcacagat gaggacatct acctgcttgg aaaagccaca ctgcctggct tccggaccat 1681 tcactgcTAG GACCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 1741 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 1801 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 1861 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 1921 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 1981 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2041 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2101 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2161 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2221 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2281 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2341 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2401 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2461 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 2521 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 2581 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 2641 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 2701 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 2761 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 2821 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 2881 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 2941 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3001 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3061 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3121 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3181 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3241 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3301 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3361 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3421 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3481 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 3541 CTTTTGCTGG CCTTTTGCTC ACATGTT //